一、中文名称：Anti-FlagTag(DYKDDDDK)抗体(M2)

二、产品描述： FLAG标签，亦称为FLAG八肽或FLAG表位，专为融合蛋白设计，且已获得专利。这种标签的重要优势在于其适用性广泛，能够在多种抗体识别实验中发挥作用。通过在目标蛋白上添加FLAG标签，即使没有特定的专用抗体，也能通过抗FLAG序列的抗体进行研究。FLAG标签的分子量相对较小，仅为1012道尔顿（Da），其多聚阴离子氨基酸成分对目标蛋白的功能影响极小。这一特性使得FLAG标签成为理想的标记工具，能够在不干扰蛋白质活性的情况下进行后续研究。此外，FLAG标签能够有效支持高特异性的下游实验（pull-down实验），从而降低非特异性背景信号，提高检测精度与可靠性。

三、产品详情：

名称：Anti-FlagTag(DYKDDDDK)抗体(M2)(RGK08501)
别名：Flagtag, Flagtag(DYKDDDDK-tag), Flag-tag, Flagpeptide(DYKDDDDK)
货号：RGK08501
宿主：小鼠
同种型：IgG1, kappa
克隆类型：单克隆
靶标：Flagtag, Flagtag(DYKDDDDK-tag), Flag-tag, Flagpeptide(DYKDDDDK)
浓度：156mg/ml
内毒素水平：请咨询实验室获取信息
纯度：95%（由SDS-PAGE确定）
纯化方式：从细胞培养上清中通过Protein A/G纯化
克隆号：M2
应用：ChIP, ELISA, EIA, FCM, ICC, IF, IP, WB
状态：液体
保存溶液：001M PBS, pH 7.4
稳定性和存储：建议使用手动解冻冰箱，避免反复冻融；短期存放（1-2周）在4°C，长期存放（12个月）在-20°C，长期存放（RGK08501）在-80°C。

四、参考文献：
Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition PMID: 33951064
An internal amino-terminal FLAG-tag octapeptide alters oligomerization of expressed surfactant protein-A PMID: 32835791
Core-Shell Magnetic Imprinted Polymers for the Recognition of FLAG-Tag peptide PMID: 36834864
Proteomics-Based Analysis of Protein Complexes in Pluripotent Stem Cells and Cancer Biology PMID: 27011181
An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments PMID: 7530459
Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5 PMID: 9238646
A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study PMID: 22465329
Tag-Specific Affinity Purification of Recombinant Proteins by Using Molecularly Imprinted Polymers PMID: 30786715
Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins PMID: 37450137
Hierarchically Imprinted Polymer for Peptide Tag Recognition Based on an Oriented Surface Epitope Approach PMID: 32990425
High-level expression of anti-FLAG tag antibody in plants PMID: 38258646
Design and characterization of stimuli-responsive FLAG-tag analogues and the illumination-induced modulation of their interaction with antibody 4E11 PMID: 16889395
Using Affinity Pulldown Assays to Study Protein-Protein Interactions of Human NEIL1 Glycosylase and the Checkpoint Protein RAD9-RAD1-HUS1 (9-1-1) Complex PMID: 37574484
Identification of Selenoprotein H Isoforms and Impact of Selenoprotein H Overexpression on Protein But Not mRNA Levels of 2 Other Selenoproteins in 293T Cells PMID: 34510207
A novel in-capillary assay for dynamically monitoring fast binding between antibody and peptides using CE PMID: 30367708
Synthesis, purification and bioactivity of recombinant human activin A expressed in the yeast Pichia pastoris PMID: 19027859
Minimal FLAG sequence useful in the functional epitope tagging of H-Ras PMID: 12074157
Lithobates catesbeianus (American Bullfrog) oocytes: a novel heterologous expression system for aquaporins PMID: 29530931
Use of azobenzene amino acids as photo-responsive conformational switches to regulate antibody-antigen interaction PMID: 19472287
Identification of amino acid residues responsible for the binding to anti-FLAG M2 antibody using a phage display combinatorial peptide library PMID: 23807489
The human D1 Dopamine receptor: heterologous expression in Saccharomyces cerevisiae and purification of the functional receptor PMID: 9631523
Tetramerization domain of human butyrylcholinesterase is at the C-terminus PMID: 9581552
Development of an efficiently cleaved, bioactive, highly pure FLAG-tagged recombinant human Mullerian Inhibiting Substance PMID: 19755162
Production and purification of human indoleamine 2,3-dioxygenase (HuIDO) protein in a baculovirus expression system and production and characterization of egg yolk antibody against the purified HuIDO PMID: 16174105
Characterization of capsid-modified adenovirus vectors containing heterologous peptides in the fiber knob, protein IX, or hexon PMID: 17006548
Topology of the Na(+)/dicarboxylate cotransporter: the N-terminus and hydrophilic loop 4 are located intracellularly PMID: 11248207

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